{"id":904,"date":"2014-01-28T15:59:50","date_gmt":"2014-01-28T22:59:50","guid":{"rendered":"http:\/\/sites.clas.ufl.edu\/macklab\/?page_id=904"},"modified":"2025-04-30T10:14:18","modified_gmt":"2025-04-30T17:14:18","slug":"protocols","status":"publish","type":"page","link":"https:\/\/macklab.nau.edu\/?page_id=904","title":{"rendered":"Protocols"},"content":{"rendered":"

Below is a list of protocols used in the Mack Lab. For specific information regarding any of these protocols, please contact Zach Madsen (zacharaih.madsen@nau.edu<\/a>).<\/p>\n

Carbon-related<\/h3>\n

Soil CO2<\/sub>\u00a0Mineralization<\/p>\n

TOC in soils and soil extracts<\/p>\n

Anthrone Assay for Reducing Sugars<\/p>\n

Total Non-structural Carbohydrate<\/p>\n

Substrate-induced Microbial Biomass C<\/p>\n

Cellulose Extraction<\/p>\n

Nitrogen-related<\/h3>\n

Ammonium Assay<\/p>\n

Reactive Ammonium Determination using Ninhydrin Method<\/p>\n

Diffusion Protocol for Determining 15N\/14N using Mass Spectrometry<\/p>\n

Nitrogen Mineralization<\/p>\n

Nitrite Assay<\/p>\n

Persulfate Oxidation for Determining DON<\/p>\n

Microbial Biomass N<\/p>\n

Phosphorus-related<\/h3>\n

Persulfate Digestion to Determine DOP and TP in water<\/p>\n

Mehlich I P<\/p>\n

Ascorbate Assay for Determining P<\/p>\n

Malachite Green Method for Determining P<\/p>\n

Sample Processing<\/h3>\n

Root Sorting<\/p>\n

Litterbag Sorting<\/p>\n

Soil Collection and Processing<\/p>\n

Dry Ash Digestion<\/p>\n

Wet Ashing Procedure<\/p>\n

Resins<\/h3>\n

Single Ion Resin Bags<\/p>\n

Mixed Bed Resin Bags<\/p>\n

Protein<\/h3>\n

Determination of Total Soluble Protein<\/p>\n

Determination of Total Free Amino Acids in Soils<\/p>\n

<\/h3>\n

Enzyme Assays<\/h3>\n

Acid Phosphatase<\/p>\n

Ammonium Oxygenase<\/p>\n

Protease Activity<\/p>\n

Denitrifying Enzyme Activity<\/p>\n

<\/h3>\n","protected":false},"excerpt":{"rendered":"

Below is a list of protocols used in the Mack Lab. For specific information regarding any of these protocols, please contact Zach Madsen (zacharaih.madsen@nau.edu). Carbon-related Soil CO2\u00a0Mineralization TOC in soils and soil extracts Anthrone Assay for Reducing Sugars Total Non-structural Carbohydrate Substrate-induced Microbial Biomass C Cellulose Extraction Nitrogen-related Ammonium Assay Reactive Ammonium Determination using Ninhydrin […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"page-full-width.php","meta":{"footnotes":""},"class_list":["post-904","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=\/wp\/v2\/pages\/904","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=904"}],"version-history":[{"count":8,"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=\/wp\/v2\/pages\/904\/revisions"}],"predecessor-version":[{"id":1817,"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=\/wp\/v2\/pages\/904\/revisions\/1817"}],"wp:attachment":[{"href":"https:\/\/macklab.nau.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=904"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}