Below is a list of protocols used in the Mack Lab. For specific information regarding any of these protocols, please contact Samantha Miller (samantha.miller@nau.edu).
Carbon-related
Soil CO2 Mineralization
TOC in soils and soil extracts
Anthrone Assay for Reducing Sugars
Total Non-structural Carbohydrate
Substrate-induced Microbial Biomass C
Cellulose Extraction
Nitrogen-related
Ammonium Assay
Reactive Ammonium Determination using Ninhydrin Method
Diffusion Protocol for Determining 15N/14N using Mass Spectrometry
Nitrogen Mineralization
Nitrite Assay
Persulfate Oxidation for Determining DON
Microbial Biomass N
Phosphorus-related
Persulfate Digestion to Determine DOP and TP in water
Mehlich I P
Ascorbate Assay for Determining P
Malachite Green Method for Determining P
Sample Processing
Root Sorting
Litterbag Sorting
Soil Collection and Processing
Dry Ash Digestion
Wet Ashing Procedure
Resins
Single Ion Resin Bags
Mixed Bed Resin Bags
Protein
Determination of Total Soluble Protein
Determination of Total Free Amino Acids in Soils
Enzyme Assays
Acid Phosphatase
Ammonium Oxygenase
Protease Activity
Denitrifying Enzyme Activity